test datasets are classified as Unassigned

[monica steffi matchado]

Dear Users/authors,

I am trying vContact2 to assign taxonomic classification for my viral contig. I was able to run the pipeline with the test dataset without any error but all the test contigs were unassigned and they are assigned as Singletons. Was these expected results? or am I doing something weird:? I used the latest db ProkaryoticViralRefSeq211-Merged .

Please help me out here.

I also ran the pipeline with my own data, again all my contigs were unassigned.

[Hamed Alahmad]

Hello,

I have the same issue!

Did you manage to solve it?

[Mengyuan Ji]

I have the same issue

[monica steffi matchado]

I tried it with my own samples and it worked fine. I did not know why the sample dataset got all unassigned results though.

[Mengyuan Ji]

My own samples showed results all as unassigned, but I knew they were indeed phage sequences. I do not know why it has to be like this. Are your contig_ids displayed normally in genome_by_genome_overview.csv?

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[monica steffi matchado]

Can you also cross check how you generate protein.translations.faa and viral_genomes_g2g.csv files? I used ProkaryoticViralRefSeq211-Merged as a reference database.

[Mengyuan Ji]

There must be something wrong because my output files are not in the right format neither the test data nor my samples.