- changed title to Use bam files of paired and unpaired (orphaned) reads
Use bam files of paired and unpaired (orphaned) reads
Can I use sorted bam
files, which mapped both paired and unpaired (orphaned) reads onto my assembly?
I have many unpaired forward short reads, where the reverse reads didn't pass QC. It's a sequencing issue, alas. BUT, I would like to include these orphaned forward reads in any way I can because my coverage is already not great.
Contigs were assembled with metaSPAdes
using both paired and unpaired simultaneously. Mapping was done with bowtie2
, which also takes in both inputs. Its output reports statistics on paired and unpaired reads in two separate categories.
Using orphaned reads doubles my coverage in many samples, and this should help contig co-occurrence calculations in metabat2
.
Comments (5)
-
reporter -
- changed status to resolved
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Please try it, it should work. This is not a support forum it is a bug/issue tracker.
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reporter - edited description
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reporter Got it. Thanks!
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