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Nuclear_Morphology / data / Nuclear statistics

These are values automatically calculated for nuclei, and displayed in the Nuclear charts tab

They are:

Statistic Dimension Formula Description
Area Area the two dimensional area of the nucleus
Perimeter Length the length of the nuclear perimeter
Max feret Length the maximum caliper diameter across the nucleus
Min diameter Length the shortest diameter through the centre of mass of the nucleus
Variability Angle the square root of the sum-of-squares difference at each index between between the nuclear profile and the dataset median profile, normalised to the length of the median profile.
Ellipticity None H/W the height divided by width of the nuclear bounding box in the vertically oriented nucleus
Aspect ratio None W/H the inverse of ellipticity
Elongation None (H-W)/(H+W) the bounding height minus the bounding width, divided by the bounding height plus the bounding width
Regularity None (piHW)/(4*area) A measure of how regular the shape is; does it have rotational symmetry
Circularity None (4*pi*area)/(perimeter^2) the closeness of the nucleus to a circle, between 0 and 1, where 1 is a perfect circle.
Bounding width Length the width of the bounding rectangle of the nucleus, after rotation to vertical
Bounding height Length the height of the bounding rectangle of the nucleus, after rotation to vertical
Angle between reference points Angle the angle between the reference point, the centre of mass, and the orientation point
Length of hook [rodent sperm only] Length the distance from the vertical region defined by the top and bottom vertical points to the lower x-edge of the bounding rectangle of the vertically oriented nucleus
Width of body [rodent sperm only] Length the distance from the vertical region defined by the top and bottom vertical points to the upper x-edge of the bounding rectangle of the vertically oriented nucleus

Parameters.png

Updated