Filtering a dataset
Filtering is a quick way to exclude cells with poor edge detection, or simply to pull out a group of interest to you. Filtering is performed from the 'Scatter' tab in the Nuclear charts panel.
Choose the parameters you want to filter on. The defaults are area and difference to the median profile; this usually shows the outsized and oddly shaped nuclei quite clearly.
The nuclei can be subdivided using the 'Filter visible' button. Zoom to a region on the chart so only a subset of the nuclei are visible, then click the 'Filter visible' button. A new child dataset will be created containing only the nuclei currently visible on the graph.