FASTQCSTATS :: [Mon Jul 2 14:55:51 2018] Name HF-2_S3_L001_R1_001_fastqc Total Sequences: 17282967 Sequence length: 51 Encoding: Sanger / Illumina 1.9 GCcontent: 45% FASTQCSTATS :: [Mon Jul 2 14:55:51 2018] Name HS-2_S2_L001_R1_001_fastqc Total Sequences: 22214785 Sequence length: 51 Encoding: Sanger / Illumina 1.9 GCcontent: 46% FASTQCSTATS :: [Mon Jul 2 14:55:51 2018] Name LS-2_S1_L001_R1_001_fastqc Total Sequences: 24203595 Sequence length: 51 Encoding: Sanger / Illumina 1.9 GCcontent: 46% cutadapt version 1.3 Command line parameters: -b AGATCGGAAGAG -m 15 -M 35 -q 0 /mnt/Raid_Dell/RNAseq.Dietas.Dmel/smRNA///HF-2_S3_L001_R1_001.fastq.gz Maximum error rate: 10.00% No. of adapters: 1 Processed reads: 17282967 Processed bases: 881431317 bp (881.4 Mbp) Trimmed reads: 5234246 (30.3%) Trimmed bases: 48722548 bp (48.7 Mbp) (5.53% of total) Too short reads: 2451 (0.0% of processed reads) Too long reads: 17239723 (99.7% of processed reads) Total time: 280.51 s Time per read: 0.016 ms === Adapter 1 === Adapter 'AGATCGGAAGAG', length 12, was trimmed 5234246 times. 34927 times, it overlapped the 5' end of a read 5199319 times, it overlapped the 3' end or was within the read No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Overview of removed sequences (5') length count expect max.err error counts 3 27692 270046.4 0 27692 4 4083 67511.6 0 4083 5 915 16877.9 0 915 6 1462 4219.5 0 1462 7 34 1054.9 0 34 8 45 263.7 0 45 9 385 65.9 0 19 366 10 212 16.5 1 178 34 11 87 4.1 1 22 65 12 12 1.0 1 0 12 Overview of removed sequences (3' or within) length count expect max.err error counts 3 235252 270046.4 0 235252 4 238190 67511.6 0 238190 5 283341 16877.9 0 283341 6 367670 4219.5 0 367670 7 343028 1054.9 0 343028 8 435679 263.7 0 435679 9 422558 65.9 0 417233 5325 10 576186 16.5 1 560907 15279 11 840052 4.1 1 815181 24871 12 779319 1.0 1 752448 26871 13 444861 1.0 1 430007 14854 14 145250 1.0 1 140317 4933 15 44689 1.0 1 43067 1622 16 27660 1.0 1 26750 910 17 7986 1.0 1 7685 301 18 628 1.0 1 570 58 19 250 1.0 1 214 36 20 987 1.0 1 930 57 21 135 1.0 1 116 19 22 2684 1.0 1 2558 126 23 123 1.0 1 109 14 24 34 1.0 1 18 16 25 17 1.0 1 10 7 26 14 1.0 1 8 6 27 6 1.0 1 5 1 28 5 1.0 1 1 4 29 4 1.0 1 2 2 30 1 1.0 1 1 31 7 1.0 1 0 7 32 50 1.0 1 1 49 33 11 1.0 1 1 10 34 84 1.0 1 0 84 35 36 1.0 1 1 35 36 71 1.0 1 0 71 37 86 1.0 1 1 85 38 251 1.0 1 95 156 39 951 1.0 1 777 174 40 215 1.0 1 129 86 41 124 1.0 1 87 37 42 108 1.0 1 12 96 43 305 1.0 1 17 288 44 99 1.0 1 18 81 45 50 1.0 1 11 39 46 32 1.0 1 14 18 47 22 1.0 1 6 16 48 24 1.0 1 8 16 49 11 1.0 1 2 9 50 173 1.0 1 1 172 cutadapt version 1.3 Command line parameters: -b AGATCGGAAGAG -m 15 -M 35 -q 0 /mnt/Raid_Dell/RNAseq.Dietas.Dmel/smRNA///HS-2_S2_L001_R1_001.fastq.gz Maximum error rate: 10.00% No. of adapters: 1 Processed reads: 22214785 Processed bases: 1132954035 bp (1133.0 Mbp) Trimmed reads: 9885901 (44.5%) Trimmed bases: 93875648 bp (93.9 Mbp) (8.29% of total) Too short reads: 2822 (0.0% of processed reads) Too long reads: 22157417 (99.7% of processed reads) Total time: 386.60 s Time per read: 0.017 ms === Adapter 1 === Adapter 'AGATCGGAAGAG', length 12, was trimmed 9885901 times. 41883 times, it overlapped the 5' end of a read 9844018 times, it overlapped the 3' end or was within the read No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Overview of removed sequences (5') length count expect max.err error counts 3 35217 347106.0 0 35217 4 4573 86776.5 0 4573 5 873 21694.1 0 873 6 541 5423.5 0 541 7 27 1355.9 0 27 8 28 339.0 0 28 9 491 84.7 0 10 481 10 70 21.2 1 29 41 11 47 5.3 1 26 21 12 16 1.3 1 2 14 Overview of removed sequences (3' or within) length count expect max.err error counts 3 351937 347106.0 0 351937 4 356252 86776.5 0 356252 5 499302 21694.1 0 499302 6 659046 5423.5 0 659046 7 662038 1355.9 0 662038 8 735463 339.0 0 735463 9 768346 84.7 0 767166 1180 10 1202148 21.2 1 1188665 13483 11 1843371 5.3 1 1821292 22079 12 1582426 1.3 1 1559883 22543 13 808370 1.3 1 797326 11044 14 237367 1.3 1 233988 3379 15 80584 1.3 1 79380 1204 16 35609 1.3 1 35056 553 17 9081 1.3 1 8918 163 18 1156 1.3 1 1083 73 19 507 1.3 1 459 48 20 2101 1.3 1 2042 59 21 313 1.3 1 285 28 22 5375 1.3 1 5271 104 23 232 1.3 1 222 10 24 46 1.3 1 33 13 25 29 1.3 1 20 9 26 23 1.3 1 21 2 27 11 1.3 1 8 3 28 4 1.3 1 4 29 4 1.3 1 2 2 30 3 1.3 1 2 1 31 4 1.3 1 2 2 32 5 1.3 1 2 3 33 4 1.3 1 0 4 34 8 1.3 1 1 7 35 13 1.3 1 0 13 36 18 1.3 1 0 18 37 27 1.3 1 0 27 38 277 1.3 1 218 59 39 1557 1.3 1 1476 81 40 300 1.3 1 264 36 41 197 1.3 1 191 6 42 75 1.3 1 45 30 43 102 1.3 1 41 61 44 53 1.3 1 26 27 45 66 1.3 1 32 34 46 48 1.3 1 25 23 47 29 1.3 1 13 16 48 30 1.3 1 5 25 49 15 1.3 1 3 12 50 46 1.3 1 12 34 cutadapt version 1.3 Command line parameters: -b AGATCGGAAGAG -m 15 -M 35 -q 0 /mnt/Raid_Dell/RNAseq.Dietas.Dmel/smRNA///LS-2_S1_L001_R1_001.fastq.gz Maximum error rate: 10.00% No. of adapters: 1 Processed reads: 24203595 Processed bases: 1234383345 bp (1234.4 Mbp) Trimmed reads: 11522659 (47.6%) Trimmed bases: 109750980 bp (109.8 Mbp) (8.89% of total) Too short reads: 2864 (0.0% of processed reads) Too long reads: 24064505 (99.4% of processed reads) Total time: 434.29 s Time per read: 0.018 ms === Adapter 1 === Adapter 'AGATCGGAAGAG', length 12, was trimmed 11522659 times. 43114 times, it overlapped the 5' end of a read 11479545 times, it overlapped the 3' end or was within the read No. of allowed errors: 0-9 bp: 0; 10-12 bp: 1 Overview of removed sequences (5') length count expect max.err error counts 3 33774 378181.2 0 33774 4 6338 94545.3 0 6338 5 1015 23636.3 0 1015 6 786 5909.1 0 786 7 117 1477.3 0 117 8 67 369.3 0 67 9 341 92.3 0 20 321 10 222 23.1 1 163 59 11 433 5.8 1 389 44 12 21 1.4 1 5 16 Overview of removed sequences (3' or within) length count expect max.err error counts 3 408171 378181.2 0 408171 4 438093 94545.3 0 438093 5 551058 23636.3 0 551058 6 662726 5909.1 0 662726 7 643244 1477.3 0 643244 8 867348 369.3 0 867348 9 1209262 92.3 0 1207364 1898 10 1653913 23.1 1 1637755 16158 11 2059251 5.8 1 2037001 22250 12 1581549 1.4 1 1560961 20588 13 828934 1.4 1 818811 10123 14 296344 1.4 1 292511 3833 15 140562 1.4 1 138679 1883 16 95764 1.4 1 94507 1257 17 32519 1.4 1 31987 532 18 1558 1.4 1 1479 79 19 519 1.4 1 444 75 20 723 1.4 1 658 65 21 230 1.4 1 206 24 22 4537 1.4 1 4446 91 23 208 1.4 1 197 11 24 57 1.4 1 39 18 25 35 1.4 1 18 17 26 15 1.4 1 14 1 27 7 1.4 1 6 1 28 11 1.4 1 7 4 29 5 1.4 1 3 2 30 2 1.4 1 1 1 31 9 1.4 1 7 2 32 7 1.4 1 0 7 33 5 1.4 1 3 2 34 4 1.4 1 0 4 35 7 1.4 1 0 7 36 4 1.4 1 0 4 37 20 1.4 1 2 18 38 275 1.4 1 226 49 39 1587 1.4 1 1550 37 40 320 1.4 1 300 20 41 193 1.4 1 183 10 42 71 1.4 1 50 21 43 90 1.4 1 47 43 44 57 1.4 1 34 23 45 41 1.4 1 18 23 46 29 1.4 1 19 10 47 28 1.4 1 13 15 48 20 1.4 1 5 15 49 21 1.4 1 6 15 50 112 1.4 1 2 110 FASTQCSTATS :: [Mon Jul 2 15:14:19 2018] Name HF-2_S3_L001_R1_001_cut_fastqc Total Sequences: 40793 Sequence length: 15-35 Encoding: Sanger / Illumina 1.9 GCcontent: 51% FASTQCSTATS :: [Mon Jul 2 15:14:19 2018] Name HS-2_S2_L001_R1_001_cut_fastqc Total Sequences: 54546 Sequence length: 15-35 Encoding: Sanger / Illumina 1.9 GCcontent: 51% FASTQCSTATS :: [Mon Jul 2 15:14:19 2018] Name LS-2_S1_L001_R1_001_cut_fastqc Total Sequences: 136226 Sequence length: 15-35 Encoding: Sanger / Illumina 1.9 GCcontent: 52%