Sometimes a certain amount of a chemical compound is added during the simulation of an experiment. This mean that the state variable of the respective compound changes it's value by a certain amount at a certain point of time.
Such event can be modeled by an additional input function:
INPUTS BolusInjection C nM conc. "bolus_amount * (1/sqrt(2*pi*injection_duration^2)) * exp(-(t - injection_timepoint)^2/(2*injection_duration^2))"
The function is a scaled normal distribution (given that
t indicates the independent variable) with area under the curve given by the value of
bolus_amount. The injection time point is given by
injection_timepoint and the duration by
Additionally, one has to specify a reaction that adds this input function to the respective state variable (here
REACTIONS -> ProteinA CUSTOM "BolusInjection"
In the folder
/Examples we provide some showcase applications
Bolus_Injection_Test that can be used as a starting point for a new modeling project.
In this example a bolus of 5 nM is injected at time point 40 minutes over a period of 1 minute, as the counter state