Similar to the bolus injection example, sometimes a washing event should be modeled.
Such event can be modeled by an additional input function:
INPUTS Washing C nM conc. "wash_strength * (1/sqrt(2*pi*wash_duration^2)) * exp(-(t - wash_timepoint)^2/(2*wash_duration^2))"
The function is a scaled normal distribution (given that
t indicates the independent variable) with area under the curve given by the value of
wash_strength and should be choosen large enough. The washing time point is given by
wash_timepoint and the duration by
Additionally, one has to specify a reaction that removes the respective state variable (here
Ligand) proportional to the
REACTIONS Ligand -> CUSTOM "Ligand * Washing"
In the folder
/Examples we provide some showcase applications
Washing_and_Injection_Test that can be used as a starting point for a new modeling project.
In this example a first bolus of 5 nM is injected at time point 30 minutes over a period of 1 minute. At time point 50 minutes
Ligand is washed almost completely, and at time point 60 minutes another bolus of 3 nM is injected.