nt.length.b Error: 2 (No such file or directory)

[Najma Al-Kharousi]

Hi,

I am using CCmetagen for the first time. The first requirement is to map sequence reads (or contigs) to the database with KMA. I applied the following command:

kma -ipe ~/path/to/sample_R1_001.fastq.gz ~/path/to/sample_R2_001.fastq.gz -o ~/Analysis/noTrimming/S56_out_kma -t_db /path/to/nt -t 8 -1t1 -mem_mode -and -apm f

Once I run I get this error:

Filename: /path/to/nt.length.b
Error: 2 (No such file or directory)

‌

I tried to read and look for a meaning of the error but I couldn’t figure it out.

I will appreciate you help please

[ptlcc]

Hi Najma

It is because the file “/path/to/nt.length.b“ does not exist. You have to alter the paths in your command so that they match up with your file locations.

Best,
Philip