R package for the automated analysis of dithionite scramblase assays.
To install the development version, you first need the devtools package.
Then you can install the flippant package using
A stable version is available from CRAN and may be installed using
A manuscript covering the package is in print at BMC Bioinflormatics and its
source provided in
Rmd form in the
inst/manuscript directory. Note that
building the manuscript takes around an hour, since it runs the whole analysis,
including code benchmarking using an
n of 100.
The original data is derived from Ploier et al. (2016; Nat Commun 7, 12832) and
contained in a
zip file in the
inst/extdata directory. Each file within the
zip archive is a text file. Lines 4 through to the penultimate line contain
tab delimited text data.
scramblase_assay_traces draws time series of relative fluorescence vs.
acquisition time, possibly split by experiment.
scramblase_assay_plot draws plots of the probability of at least one flippase
vs. the protein to phospholipid ratio.
scramblase_assay_stats calculates the fit constant for each experiment.
scramblase_assay_calculations provides a lower-level overview of model fit