# WARNING before you start
# Install this tool on a private Galaxy ONLY
# Please NEVER on a public or production instance
This is an unusual Galaxy tool that generates very simple new Galaxy tools that run the user
a supplied script (R, python, perl, bash...) over a single input file.
Whenever you run this tool, the ToolFactory, you should have prepared a script to paste into a text box,
and a small test input example ready to select from your history to test your new script
If the script runs sucessfully, a new Galaxy tool that runs your script can be generated.
The new tool is in the form of a special new Galaxy datatype - toolshed.gz - as the name suggests,
it's an archive ready to upload to a Galaxy ToolShed as a new tool repository.
Once it's in a ToolShed, it can be installed into any local Galaxy server from
the server administrative interface.
Once your new tool is installed, local users can run it - each time, the script that was supplied
when it was built will be executed with the input chosen from the user's history. In other words,
the tools you generate with the ToolFactory run just like any other Galaxy tool,
but run your script every time.
*Reasons to read further*
If you use Galaxy to support your research;
You and fellow users are sometimes forced to take data out of Galaxy, process it with ugly
little perl/awk/sed/R... scripts and put it back;
You do this when you can't do some transformation in Galaxy (the 90/10 rule);
You don't have enough developer resources for wrapping dozens of even relatively simple tools;
Your research and your institution would be far better off if those feral scripts were all tucked safely in
your local toolshed and Galaxy histories.
*The good news* If it can be trivially scripted, it can be running safely in your
local Galaxy via your own local toolshed in a few minutes - with functional tests.
*Value proposition* The ToolFactory allows Galaxy to efficiently take over most of your lab's dark script matter,
making it reproducible in Galaxy and shareable through the ToolShed.
That's what this tool does. You paste a simple script and the tool returns
a new, real Galaxy tool, ready to be installed from the local toolshed to local servers.
Scripts can be wrapped and online literally within minutes.
*To fully and safely exploit the awesome power* of this tool, Galaxy and the ToolShed,
you should be a developer installing this tool on a private/personal/scratch local instance where you are an admin_user.
Then, if you break it, you get to keep all the pieces
** Installation **
This is a Galaxy tool. You can install it most conveniently using the administrative "Search and browse tool sheds" link.
Find the Galaxy Test toolshed (not main) and search for the toolfactory repository.
Open it and review the code and select the option to install it.
If you can't get the tool that way, the xml and py files here need to be copied into a new tools subdirectory such as tools/toolfactory
Your tool_conf.xml needs a new entry pointing to the xml file - something like::
<section name="Tool building tools" id="toolbuilders">
If not already there (I just added it to datatypes_conf.xml.sample), please add:
<datatype extension="toolshed.gz" type="galaxy.datatypes.binary:Binary" mimetype="multipart/x-gzip" subclass="True" />
to your local data_types_conf.xml.
Ensure that html sanitization is set to False and uncommented in universe_wsgi.ini
You'll have to restart the server for the new tool to be available.
Of course, R, python, perl etc are needed on your path if you want to test scripts using those interpreters.
Adding new ones to this tool code should be easy enough. Please make suggestions as bitbucket issues and code.
The HTML file code automatically shrinks R's bloated pdfs, and depends on ghostscript. The thumbnails require imagemagick .
* Restricted execution *
The new tool factory tool will then be usable ONLY by admin users - people with IDs in admin_users in universe_wsgi.ini
**Yes, that's right. ONLY admin_users can run this tool** Think about it for a moment. If allowed to run any
arbitrary script on your Galaxy server, the only thing that would impede a miscreant bent on destroying all your
Galaxy data would probably be lack of appropriate technical skills.
*What it does* This is a tool factory for simple scripts in python, R and perl currently.
Functional tests are automatically generated. How cool is that.
LIMITED to simple scripts that read one input from the history.
Optionally can write one new history dataset,
and optionally collect any number of outputs into links on an autogenerated HTML
index page for the user to navigate - useful if the script writes images and output files - pdf outputs
are shown as thumbnails and R's bloated pdf's are shrunk with ghostscript so that and imagemagik need to
Generated tools can be edited and enhanced like any Galaxy tool, so start small and build up since
a generated script gets you a serious leg up to a more complex one.
*What you do* You paste and run your script
you fix the syntax errors and eventually it runs
You can use the redo button and edit the script before
trying to rerun it as you debug - it works pretty well.
Once the script works on some test data, you can
generate a toolshed compatible gzip file
containing your script ready to run as an ordinary Galaxy tool in a
repository on your local toolshed. That means safe and largely automated installation in any
production Galaxy configured to use your toolshed.
*Generated tool Security* Once you install a generated tool, it's just
another tool - assuming the script is safe. They just run normally and their user cannot do anything unusually insecure
but please, practice safe toolshed.
Read the fucking code before you install any tool.
Especially this one - it is really scary.
If you opt for an HTML output, you get all the script outputs arranged
as a single Html history item - all output files are linked, thumbnails for all the pdfs.
Ugly but really inexpensive.
Patches and suggestions welcome as bitbucket issues please?
long route to June 2012 product
derived from an integrated script model
Note to the unwary:
This tool allows arbitrary scripting on your Galaxy as the Galaxy user
There is nothing stopping a malicious user doing whatever they choose
Totally insecure. So, trusted users only
copyright ross lazarus (ross stop lazarus at gmail stop com) May 2012
all rights reserved
Licensed under the LGPL if you want to improve it, feel free https://bitbucket.org/fubar/galaxytoolfactory/wiki/Home
Material for our more enthusiastic and voracious readers continues below - we salute you.
**Motivation** Simple transformation, filtering or reporting scripts get written, run and lost every day in most busy labs
- even ours where Galaxy is in use. This 'dark script matter' is pervasive and generally not reproducible.
**Benefits** For our group, this allows Galaxy to fill that important dark script gap - all those "small" bioinformatics
tasks. Once a user has a working R (or python or perl) script that does something Galaxy cannot currently do (eg transpose a
tabular file) and takes parameters the way Galaxy supplies them (see example below), they:
1. Install the tool factory on a personal private instance
2. Upload a small test data set
3. Paste the script into the 'script' text box and iteratively run the insecure tool on test data until it works right -
there is absolutely no reason to do this anywhere other than on a personal private instance.
4. Once it works right, set the 'Generate toolshed gzip' option and run it again.
5. A toolshed style gzip appears ready to upload and install like any other Toolshed entry.
6. Upload the new tool to the toolshed
7. Ask the local admin to check the new tool to confirm it's not evil and install it in the local production galaxy
**Simple examples on the tool form**
A simple Rscript "filter" showing how the command line parameters can be handled, takes an input file,
does something (transpose in this case) and writes the results to a new tabular file::
# transpose a tabular input file and write as a tabular output file
ourargs = commandArgs(TRUE)
inf = ourargs
outf = ourargs
inp = read.table(inf,head=F,row.names=NULL,sep='\t')
outp = t(inp)
write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=F)
Calculate a multiple test adjusted p value from a column of p values - for this script to be useful,
it needs the right column for the input to be specified in the code for the
given input file type(s) specified when the tool is generated ::
# use p.adjust - assumes a HEADER row and column 1 - please fix for any real use
column = 1 # adjust if necessary for some other kind of input
fdrmeth = 'BH'
ourargs = commandArgs(TRUE)
inf = ourargs
outf = ourargs
inp = read.table(inf,head=T,row.names=NULL,sep='\t')
p = inp[,column]
q = p.adjust(p,method=fdrmeth)
newval = paste(fdrmeth,'p-value',sep='_')
q = data.frame(q)
names(q) = newval
outp = cbind(inp,newval=q)
write.table(outp,outf, quote=FALSE, sep="\t",row.names=F,col.names=T)
Another Rscript example without any input file - generates a random heatmap pdf - you must make sure the option to create an HTML output file is
turned on for this to work. The heatmap will be presented as a thumbnail linked to the pdf in the resulting HTML page::
# note this script takes NO input or output because it generates random data
foo = data.frame(a=runif(100),b=runif(100),c=runif(100),d=runif(100),e=runif(100),f=runif(100))
bar = as.matrix(foo)
pdf( "heattest.pdf" )
A Python example that reverses each row of a tabular file. You'll need to remove the leading spaces for this to work if cut
and pasted into the script box. Note that you can already do this in Galaxy by setting up the cut columns tool with the
correct number of columns in reverse order,but this script will work for any number of columns so is completely generic::
# reverse order of columns in a tabular file
inp = sys.argv
outp = sys.argv
i = open(inp,'r')
o = open(outp,'w')
for row in i:
rs = row.rstrip().split('\t')
**Attribution** Copyright Ross Lazarus (ross period lazarus at gmail period com) May 2012
All rights reserved.
Licensed under the LGPL