minlen capability with MaskPrimers
Issue #12
resolved
A minlen capability added to maskPrimers-align would 1 - increase speed ? 2 - obviate some post-processing
Use case: Single cell data uses mixed-length barcodes, to increase the barcode space and add nucleotide diversity (for the sequence). The barcode is called by aligning a long constant sequence that is 3' in the read with maskPrimers-align+cut, then I post-process results with a one-liner to filter for barcodes of a certain length... which works fine but involves non-pRESTO code. just a suggestion; not high priority at al.
Comments (3)
-
-
-
assigned issue to
Jason Vander Heiden
- edited description
-
assigned issue to
-
- changed status to resolved
Probably accomplished by either the new MaskPrimers-extract command and/or padding primer sequences with Ns.
- Log in to comment
Had already intended to add this at some point.