Replace usearch with an open source tool in AssemblePairs-reference
vsearch doesn't provide local alignment methods, so we cannot replace usearch with vsearch in AssemblePairs-reference. We need an alternative.
Binaries for BWA and swipe and linux only, so that unfortunately invalidates them. Perhaps the SSW implementation in scikit-bio?
Comments (4)
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reporter -
reporter Added blastn support in 9c90566. usearch and blastn do not yield the same results, apparently because blastn is being more strict returning hits for some unknown reason.
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reporter Syncing the word size and masking between ublast and blastn seems to have resolved most of the differences. Still some testing to do, but I'm going to go with the blastn solution for now.
Dockerfiles have also been updated with blast+ installs.
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reporter - changed status to resolved
Seems to work fine as of 5102751. There are minor differences between the usearch and blastn alignments, but this may be impractical to resolve (1 indel problem out of 100 alignments).
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