paired-end sample
I have some confusion for the parameter setting of the mageck-vispr, according to config.yaml template, I input the paired-end fastq file as :
samples:
Pos:
- qc_fastqs/Pos_FKDL202624913-1a-A8_qc_1.fastq.gz
- qc_fastqs/Pos_FKDL202624913-1a-A8_qc_2.fastq.gz
But, in the log of mageck, the first line is 'INFO @ Fri, 15 Jan 2021 12:27:04: Parameters: /software/anaconda3/bin/mageck count --output-prefix results/count/all --norm-method control --list-seq ../library_b.txt --fastq qc_fastqs/Pos_FKDL202624913-1a-A8_qc_1.fastq.gz,qc_fastqs/Pos_FKDL202624913-1a-A8_qc_2.fastq.gz --sample-label Pos --count-pair False --control-sgrna ../library_b_control.txt --trim-5 24 '. It looks like mageck-vispr have identified the two files as technical duplicates rather than paired-end files, did I enter the wrong parameter?