R kernel crashes while loading R package

Issue #595 resolved
Felix Kögel created an issue

Hello,

First of all, I’m new to rpy2 / jupyter so please don’t judge me if this isn’t the correct place to ask my question.

I am trying to set up an integrated workflow for data analysis using R and Python and I encounter the following error:

I am on Ubuntu 19.04. running a conda environment using Jupyter 1.0.0, Python 3.7.4, R 3.5.1, r-irkernel 1.0.2 and rpy2 3.1.0 and I installed the R-package Seurat through R.

When I create a Jupyter notebook using the R-kernel, I can load Seurat with library(Seurat) just fine.

I can also use R code in python using rpy2 and the rmagic such as:

%load_ext rpy2.ipython
%%R
data(allen, package = 'scRNAseq')
adata_allen <- as(allen, 'SingleCellExperiment')

However when I try to load Seurat using rpy2 the kernel crashes:

%%R
library(Seurat)

And I get the following message:

Jupyter gives the following message in the command line:

[I 16:39:01.388 NotebookApp] KernelRestarter: restarting kernel (1/5), keep random ports
kernel 23284ec0-63d5-4b61-9ffa-b52d19851eab restarted

Note that other libraries such as library(dplyr) load just fine using rpy2.

My complete conda environment can be found in the attached text file.

I just can’t seem to figure out what is causing the problem. Is there a way to get a more verbose error message from Jupyter?

Your help would be greatly appreciated!

Regards Felix

Comments (3)

  1. Felix Kögel reporter

    As lgautier pointed out on my post on stackoverflow, there seems to be some kind of problem with reticulate.

    Following advice from this thread, I was able to solve my problem by downgrading to Seurat 3.0.2. As far as I understand, the initial problem is still an open but, but at least this workaround works well for me!

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