Use two B-factors for "Relioncor"?

[Oliver Clarke]

Hi,

Would it be possible to implement the use of two different B-factors (one for whole frame alignment and one for patch-based alignment) in the Relion implementation of Motioncor2?

The idea is to use a higher B-factor (e.g. 500) to effectively wright alignment towards lower spatial frequencies for global whole-frame alignment, and a lower B-factor (e.g. 150) for patch-based alignment.

This has been added to recent versions of Motioncor2, and it seems to help avoid problematic "double vision" errors like the attached, where I am assuming the alignment gets stuck in a false minimum during global alignment and then cannot recover during patch-based alignment.

Cheers Oli

[Takanori Nakane]

Hi, this is an interesting idea. Can you share me a movie that causes this problem?

[Oliver Clarke]

Sure thing Takanori! I will find a movie that gives this behavior with relioncor but works with the current version of MC2 (the pictured one will likely do the trick)

Cheers Oli