Is single-scaffold virus strictly the required input format for iphop?

[Zhichao Zhou]

Is single-scaffold virus strictly the required input format for iphop?

Now I linked multiple fasta of one virus with Ns to make a single-scaffold virus. However, this will introduce some different ORF predictions. Will this influence the host prediction result?

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[Simon Roux]

This is correct, single-scaffold virus is currently the required input. Stitching together multiple scaffolds into a single virus sequence has not been tested, and would indeed likely influence the prediction result.

[Simon Roux]

Answered