bins to large in the output of metabat2

[Former user]

Hello, the output of metabat2 generates some very large bins, on the order of 11 and 30 kbp. I suspect they are composites of mixed heterogeneous strains. Is there a way to debug these bins?

[Zhong Wang]

Hi,

I would suggest using the CheckM tool to check strain heterogeneity (https://ecogenomics.github.io/CheckM/). There is also a checkM narrative available at Kbase: https://kbase.us/n/33233/351/#step6

There could be several alternative explanations for very large bins, such as Eukaryotes, poor assembly, poor binning, etc.

Hope this helps.

Best,

Zhong

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