question : binning with multiple contigs files

[Meijun Wu]

Hello,

I have generate 3 contigs.fa for 3 different samples, i have generate also the corresponding bam files. i don’t know if metahit2 is capable to treat multiple contigs at once. (when i tried put multiple files in the commande it don’t work).

please give some advances please, please.

thank you.

[Rob Egan]

Hi Meijun,

Please read the papers that describe what metabat can do. It is designed to bin together contigs from a single assembly. If you perform an assembly of the 3 samples together (i.e. a combined assembly) and then create the 3 BAM files for the different samples, mapped against that single assembly, then it will work as designed and provide meaningful results.

Thanks,

Rob

[Meijun Wu]

Thank you for your advices (sorry for the wrong spelling)