Error bowtie1index and bowtie2index

Issue #147 resolved
Former user created an issue

I can't work out why I see this error. I also see it with example ini files so I think I'm doing something fundamentally wrong.

perl ./miARma config/config_v0.2.ini -check

gives:

ERROR Please check that the parameter fasta inside section [Aligner] is correct.

[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA, miRDeep or Bowtie1-Bowtie2)
aligner=Bowtie2
; Path of the genomic fasta sequence to build the index
fasta=genomes/human/Bowtie2/genome.fa
;Name to write in the index files
indexname=genome
; #Max # mismatches in seed alignment in bowtie analysis (0-1)
bowtiemiss=0
; #Length of seed substrings in bowtie analysis (>5, <32)
bowtieleng=19

I also tried Bowtie 1:

[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA or miRDeep)
aligner=Bowtie1
; Bowtie 2 index
bowtie1index=/home/miARma/miARma/genomes/human/BowtieIndex

And two:

[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA or miRDeep)
aligner=Bowtie2
; Bowtie 2 index
bowtie2index=/home/miARma/miARma/genomes/human/Bowtie2Index

with ERROR Please check that the parameter bowtie1index inside section [Aligner] is correct. and ERROR Please check that the parameter bowtie2index inside section [Aligner] is correct. relatively

Comments (2)

  1. Eduardo Andres Leon

    For indexes you have to write the name of the prefix name. That is the filename of the indexes without the extensions (bw2, eb …)

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