Error bowtie1index and bowtie2index
Issue #147
resolved
I can't work out why I see this error. I also see it with example ini files so I think I'm doing something fundamentally wrong.
perl ./miARma config/config_v0.2.ini -check
gives:
ERROR Please check that the parameter fasta inside section [Aligner] is correct.
[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA, miRDeep or Bowtie1-Bowtie2)
aligner=Bowtie2
; Path of the genomic fasta sequence to build the index
fasta=genomes/human/Bowtie2/genome.fa
;Name to write in the index files
indexname=genome
; #Max # mismatches in seed alignment in bowtie analysis (0-1)
bowtiemiss=0
; #Length of seed substrings in bowtie analysis (>5, <32)
bowtieleng=19
I also tried Bowtie 1:
[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA or miRDeep)
aligner=Bowtie1
; Bowtie 2 index
bowtie1index=/home/miARma/miARma/genomes/human/BowtieIndex
And two:
[Aligner]
; Aligner (Bowtie1, Bowtie2, BWA or miRDeep)
aligner=Bowtie2
; Bowtie 2 index
bowtie2index=/home/miARma/miARma/genomes/human/Bowtie2Index
with
ERROR Please check that the parameter bowtie1index inside section [Aligner] is correct.
and
ERROR Please check that the parameter bowtie2index inside section [Aligner] is correct.
relatively
Comments (2)
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- changed status to resolved
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For indexes you have to write the name of the prefix name. That is the filename of the indexes without the extensions (bw2, eb …)