Change in feature ?

[Eduardo Andres Leon]

Typically, there will be reads that cannot be uniquely assigned to a gene, either because the read was aligned to multiple locations (multi-reads) or the read’s position is annotated as part of several overlapping features. For the purpose of calling differential expression, such reads should be discarded

[Eduardo Andres Leon]

After some tests, -M and -O has been removed. It can be included using : parameters=-M -O