Known miRNA identificaton

[Former user]

Hi,

For Identifying known miRNAs should we have to provide mature region or hairpin loop region in gtf for subread? And what is the ideal way?

Regards, Manoharan

[Eduardo Andres Leon]

Dear Manoharan, This mainly depends on your experiment. You can provide miRNA genes (pre-miRNAs, harping loop …) , and you will see the expression of the whole gene. If you want to measure the expression of “miRNA transcripts” , such as -3p or 5p, then you will have to provide mature region coordinates

Edu

[Manoharan]

Dear Eduardo,

Thanks, Is "significant randfold p-value" in miRDeep_results excel files has been filtered for significant randfold p-value(If we run separately it provides yes which is for significant randfold)?.

Is there a miARma version which provides pdf/html files as well. I tried modifying the Aligner.pm It is giving error for me.

Regards,

[Eduardo Andres Leon]

Duplicate of #61.