Known miRNA identificaton
Issue #67
duplicate
Hi,
For Identifying known miRNAs should we have to provide mature region or hairpin loop region in gtf for subread? And what is the ideal way?
Regards, Manoharan
Comments (3)
-
-
Dear Eduardo,
Thanks, Is "significant randfold p-value" in miRDeep_results excel files has been filtered for significant randfold p-value(If we run separately it provides yes which is for significant randfold)?.
Is there a miARma version which provides pdf/html files as well. I tried modifying the Aligner.pm It is giving error for me.
Regards,
-
- changed status to duplicate
Duplicate of #61.
- Log in to comment
Dear Manoharan, This mainly depends on your experiment. You can provide miRNA genes (pre-miRNAs, harping loop …) , and you will see the expression of the whole gene. If you want to measure the expression of “miRNA transcripts” , such as -3p or 5p, then you will have to provide mature region coordinates
Edu