Proble running iDiffir

[Former user]

Hi,

I am a bench researcher trying to identify intron retention events in my rna-seq experiments (Arabidopsis mutant versus wild-type).

I have correctly installed all the required packages and the iDiffir. I got the depths and when I type: idiffir.py -l Mutant Wildtype -o my_result TAIR10_GFF3_genes.gff mt_rep1_depths wt_rep1_depths

I get this: root@gdaras-Z68X-UD3P-B3:/home/gdaras/Επιφάνεια εργασίας/iDiffIR_v0.3.1# idiffir.py -l Mutant Wildtype -o my_result TAIR10_GFF3_genes.gff mt_rep1_depths wt_rep1_depths Traceback (most recent call last): File "/usr/local/bin/idiffir.py", line 22, in <module> from iDiffIR.Plot import * File "/usr/local/lib/python2.7/dist-packages/iDiffIR/Plot.py", line 114 ofile.write('\t'.join(['geneID', 'lowExonCoords', ^ IndentationError: expected an indented block

Any advice? Your help will be really appreciated!

Gerasimos Daras, PhD Agricultural University of Athens Laboratory of Molecular Biology Athens, Greece

[Mike Hamilton]

Hi, It appears that you are using a very old version of iDiffIR. Please download a fresh copy bitbucket.