• Download pyinstaller 1.5 from http://www.pyinstaller.org/

• First, unpack the archives (pyinstaller-1.5.zip and ApicoAMPxx.zip) on your path of choice. (Installer is not a Python package, so it doesn't need to go in site-packages)

• Open the Terminal app, change directories to the location of "pyinstaller-1.5", and type the following commands in order:

  ```python Configure.py```
  

  python Makespec.py --onefile pathToApicoAMP/ApicoAMP_GUI.py

Note: pathToApicoAMP in this command should be replaced by the path ApicoAMP_GUI.py resides. This command will prepare a spec file, and the path/name of the prepared spec file will be printed to the screen, this path/name should be provided to the next command

• Before running the next command, add the following lines at the end of the spec file prepared with the previous command:

import sys
if sys.platform.startswith("darwin"):
    app = BUNDLE(exe,
                 name=os.path.join('dist', 'NAME.app'),
                 version=version)

and run the following command:

```python Build.py pathToSpecfile/specfile```

Note: pathToSpecfile/specfile in this command should be replaced by the path/name of the spec file prepared by the last command. These commands should create a folder under "pyinstaller-1.5" folder named "ApicoAMP". Under "ApicoAMP/dist" folder, ApicoAMP.app should reside. Copy this file under the folder "ApicoAMP" (in which ApicoAMP_GUI.py resides) and launch it from there.

Please cite the following paper if you are using this tool:

Cilingir, Gokcen, Audrey OT Lau, and Shira L. Broschat. "ApicoAMP: The first computational model for identifying apicoplast-targeted transmembrane proteins in Apicomplexa." Journal of microbiological methods 95.3 (2013): 313-319.