Error in Differential Expression module using EdgeR
Dear all, I am new in using miarma, so I apologize in advance if I'm unable to accurately express my issue. I'm trying the mRNA basic example using hisat2 index. I get the following error in Differential Expression module using EdgeR:
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miARma, miRNA and RNASeq Multiprocess Analysis
miARma v 1.7.2 (Dec-2017)
Created at Computational Biology and Bioinformatics Group (CbBio)
Institute of Biomedicine of Seville. IBIS (Spain)
Modified and Updated at Bioinformatics Unit at IPBLN-CSIC
Institue for Parasitology and Biomedicine Lopez-Neyra (IPBLN-CSIC).
Granada (Spain)
Copyright (c) 2017 IBIS & IPBLN. All rights reserved.
mail : miARma-devel@cbbio.es
[Thu Dec 28 12:22:22 2017] Starting a miARma analysis for mRNA [Thu Dec 28 12:22:22 2017] Checking provided parameters for: Quality,Aligner,ReadCount,DEAnalysis,FAnalysis,TargetPrediction. [Thu Dec 28 12:22:22 2017] Checking General-output_dir parameter ... Exists! [Thu Dec 28 12:22:22 2017] The folder specified in (output_dir=Examples/basic_examples/mRNAs/results/) already exists. [Thu Dec 28 12:22:22 2017] Wait 5 seconds to overwrite the folder. Cancel otherwise: 0 [Thu Dec 28 12:22:28 2017] Continue. [Thu Dec 28 12:22:28 2017] All parameters are correct. [Thu Dec 28 12:22:28 2017] Starting Quality Analysis. [Thu Dec 28 12:22:34 2017] Quality Analysis finished. [Thu Dec 28 12:22:34 2017] No processed files are found [neither cutadapt, nor reaper nor adaptrimming folders], assuming Examples/basic_examples/mRNAs/reads_test5/ are already processed [Thu Dec 28 12:22:34 2017] Starting a "Hisat2" Alignment Analysis [Thu Dec 28 12:25:48 2017] "Hisat2" Alignment Analysis finished [Thu Dec 28 12:25:48 2017] Starting a Readcount Analysis [Thu Dec 28 12:26:28 2017] Readcount Analysis finished. [Thu Dec 28 12:26:28 2017] Starting a differential expression analysis using EdgeR-Noiseq software(s) Problem while running this R command: print(resultsfiles)
Error: print(resultsfiles) : object 'resultsfiles' not found Execution halted
I have already read the Issue #63 and all the R required packages are correctly installed.
My R version is 3.4.3
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Could you send me your ini + log files (both please, the one called miARma_stat.log and the miARma.log). Cheers