GlycoStore is a curated chromatographic, electrophoretic and mass-spectrometry composition database of N-, O-, glycosphingolipid (GSL) glycans and free oligosaccharides associated with a range of glycoproteins, glycolipids and biotherapeutics. The database is built on publicly available experimental data sets from GlycoBase developed in the Oxford Glycobiology Institute and then the National Institute for Bioprocessing Research and Training (NIBRT). It has now been extended to include recently published and in-house data collections from the Bioprocessing Technology Institute (BTI) A*STAR, Macquarie University and Ludger Ltd. GlycoStore provides access to approximately 850 unique glycan structure entries supported by over 10000 retention positions determined by: 1) hydrophilic interaction chromatography (HILIC) ultra-high performance liquid chromatography (U/HPLC) and reversed phase (RP)-U/HPLC with fluorescent detection; 2) porous graphitized carbon (PGC) chromatography in combination with ESI-MS/MS detection; and 3) capillary electrophoresis with laser induced fluorescence detection (CE-LIF). GlycoStore enhances many features previously available in GlycoBase while addressing the limitations of the data collections and model of this popular resource. GlycoStore aims to support detailed glycan analysis by providing a resource that underpins current workflows. It will be regularly updated by by expert annotation of published data and data obtained from the project partners.
This code repository is being developed for the migration of GlycoStore and UniCarbKB to a RDF format (based on GlycoRDF). The UniCarbKB section is under active development and relies on the existing data model, whilst GlycoStore is in production. All major classes have been annotated and developers can query the data using the data model Examples.
- Matthew Campbell - Griffith UniversityInstitute for Glycomics
- Sophie Zhao - BTI, A*STAR Singapore
- Ian Walsh - BTI, A*STAR Singapore
- Jodie Abrahams - Institute for Glycomics, Griffith University
- Louise Royle - Ludger
- Pauline Rudd - NIBRT and BTI, A*STAR Singapore
- Terry Nguyen-Khuong - BTI, A*STAR Singapore)
- Nicki Packer - Institute for Glycomics, Griffith University
- Daniel Spencer and Daryl Fernandes - Ludger
- Andras Guttman - HBLS
Also acknowledge the support of Nobu Aoki (Soka University) for discussions and help with Spring Framework, and members of http://glic.glycoinfo.org.
We also acknowledge the massive efforts of the original GlycoBase team who seeded this project.
The code is licensed under GNU AGPI refer to license.txt and file headers. Please note that currently the data is covered by the Creative Commons License as per web site (https://www.glycostore.org).