Welcome to the wiki for Nuclear Morphology Analysis. This software, either standalone or as a plugin for ImageJ, allows for automated detection of round or asymmetric nuclei in images, and subsequent morphological analysis. It can specifically recognise features in mouse and pig sperm nuclei, but any shapes will be processed.
The program provides options to separate heterogeneous populations via clustering on nuclear parameters (including the shape profiles), detection of FISH signals for nuclear organisation studies, and nuclear cartograpy for dynamic warping of FISH signals.
The analysis method is described in Skinner et al, 2019: A high-throughput method for unbiased quantitation and categorisation of nuclear morphology. Biol Reprod. doi:10.1093/biolre/ioz013.
How to perform basic tasks
- Creating a new analysis
- Open a saved dataset
- Filter the cells in a dataset
- Manually curate the cells in a dataset
- Detecting FISH signals
- Warping FISH signals
- Post-FISH to pre-FISH mapping
- Updating profile segments
- Updating individual cell data
- Exporting raw data to a file
- Migrating data between versions
Guide to the interface