Welcome to the wiki for Nuclear Morphology Analysis. This software allows for automated detection of round or asymmetric nuclei in images, and subsequent morphological analysis. It come with rules to recognise features in mouse and pig sperm nuclei, but can be extended to detect custom shapes.
We can use the software to separate heterogeneous populations via clustering on nuclear parameters (including their shapes), detection of FISH signals for nuclear organisation studies, and dynamic warping of FISH signals to understand the average position of signal in a population.
If you use this, please cite our paper Skinner et al, 2019: A high-throughput method for unbiased quantitation and categorisation of nuclear morphology. Biol Reprod. doi:10.1093/biolre/ioz013.
How to perform basic tasks
A more detailed user guide is included with Nuclear Morphology Analysis - open it with
Help > Open user guide
- Running a new analysis
- Curating and filtering nuclei
- Detecting FISH signals
- Warping FISH signals
- Exporting raw data
Guide to the interface